Description
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IAPP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to IAPP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IAPP and unlabeled IAPP (Standards or samples) with the pre-coated antibody specific to IAPP. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IAPP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IAPP in the sample